Depth-resolved fluorescence spectroscopy reveals layered structure of tissue

Depth-resolved fluorescence spectroscopy reveals layered structure of tissue.

A confocal fluorescence spectroscopy system is instrumented to study depth-resolved autofluorescence in biological tissue. The system provides the capability of optical sectioning with the maximal detectiondepth up to 120 m in the examined tissue samples. It was found that the topmost keratinizing epithelial layer produces strong fluorescence similar to collagen. The fluorescence signal from ep...

Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue.

A portable confocal system with the excitations at 355nm and 457nm was instrumented to investigate the depth-resolved fluorescence of cervical tissue. The study focused on extracting biochemical and morphological information carried in the depth-resolved signals measured from the normal squamous epithelial tissue and squamous intraepithelial lesions. Strong keratin fluorescence with the spectra...

Combined depth- and time-resolved autofluorescence spectroscopy of epithelial tissue.

A time-resolved confocal fluorescence spectroscopy system is built to measure the fine structure and localized biochemistry of epithelial tissue. It is found that the autofluorescence excited at 405 nm is sensitive to the cellular metabolism and can be used to sense the metabolic status of epithelial tissue. The decay of autofluorescence excited at 405 nm can be accurately fitted with a dual-ex...

Time-resolved fluorescence spectroscopy

Time resolved Raman spectroscopy for depth analysis of multi - layered mineral samples Chapter 3 3

Time Resolved Raman Spectroscopy (TRRS) can provide subsurface information from multi-layered samples of transparent and translucent evaporative and silicate minerals up to several cm thick. Depth information was obtained using 3-ps pulsed laser excitation at 720 nm and a gated intensified CCD detector with stepwise increasing delay times. Blocks of different minerals were used as first, second...